Fig. 4

Amplification of the NS5 coding region of JEV from infected cells. NS5 coding sequences were amplified by PCR of cDNA templates derived from infected cells. Mosquito cell line (C6/36) was infected with JEV porcine vaccine strain at a multiplicity of infection of 0.2, 0.02, or 0.002. After 24 or 48 h, infected cells were collected and prepared for RT-PCR (see “Materials and Methods”). Mock: mock-infected cells (cells inoculated with PBS); NC: negative control (distilled water); PC: positive control (the genome of Dengue virus 2 PDK53, accession KU725664 [as used in Fig. 3]). The bands represent amplicons of the NS5 region (upper panel) and actin (lower panel). The images shown are cropped from the whole gel images