Fig. 3
From: Development of genus-specific universal primers for the detection of flaviviruses
PCR assay using the universal primer pair to amplify the NS5 region. Artificially synthesized oligonucleotides encoding flavivirus NS5 sequences (see “Materials and methods”) were used as templates for the PCR assay. The viral strains detected in this assay are as follows: DENV: Dengue virus 2 PDK53 (accession KU725664); ZIKV: Zika virus mosquito/Haiti/1682/2016 (accession MF384325); JEV: Japanese encephalitis virus Nakayama/MY/2009/P578662 (accession HE861351) (A); WNV: West Nile virus BSL26-11 (accession JQ700442); YFV: YFV ES-504 (accession KY885000); TBEV: Tick-borne encephalitis virus C11-13 (accession KP644245) (B); Aedes flavivirus: Aedes flavivirus Narita-21 (accession AB488408); and Culex flavivirus: Culex flavivirus Tokyo (accession AB262759) (C). Lane 1, FU1/cFD2; lane 2, MA/cFD2; lane 3, EMF1/VD8; lane 4, FUDJ9166/CFDJ9977; lane 5, Pan-flavivirus FW/RV (this article). NC: negative control (distilled water). The bands represent amplicons of the NS5 region. The images shown are cropped from the whole gel images