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Table 2 Validation parameters for mRNA quantification of segments 1, 4, and 5 by strand-specific real-time RT-PCR using tagged primers

From: Development of a method for evaluating the mRNA transcription activity of influenza virus RNA-dependent RNA polymerase through real-time reverse transcription polymerase chain reaction

Target Sensitivity (copies) Linear regression
  Slope Intercept Amplification efficiency (E%) R2
Segment 1 mRNA 101  − 3.2232 38.833 100 0.9979
Segment 4 mRNA 101  − 3.1873 38.24 100 0.9967
Segment 5 mRNA 101  − 3.2491 39.379 100 0.9972