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Table 2 Validation parameters for mRNA quantification of segments 1, 4, and 5 by strand-specific real-time RT-PCR using tagged primers

From: Development of a method for evaluating the mRNA transcription activity of influenza virus RNA-dependent RNA polymerase through real-time reverse transcription polymerase chain reaction

Target

Sensitivity (copies)

Linear regression

 

Slope

Intercept

Amplification efficiency (E%)

R2

Segment 1

mRNA

101

 − 3.2232

38.833

100

0.9979

Segment 4

mRNA

101

 − 3.1873

38.24

100

0.9967

Segment 5

mRNA

101

 − 3.2491

39.379

100

0.9972

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Virology Journal

ISSN: 1743-422X