Fig. 2From: Development of a method for evaluating the mRNA transcription activity of influenza virus RNA-dependent RNA polymerase through real-time reverse transcription polymerase chain reactionEffect of the reaction time on the influenza virus mRNA synthesis. The RNA polymerase reaction mixture containing 5 mM of MgCl2 was incubated for 0, 15, 30, 60, and 120 min at 37 °C. a Segment 1 mRNA, b segment 4 mRNA, and c segment 5 mRNA. Data are presented as mean ± standard deviation (n = 3)Back to article page