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Fig. 3 | Virology Journal

Fig. 3

From: Antiviral activity of diallyl trisulfide against H9N2 avian influenza virus infection in vitro and in vivo

Fig. 3

Effect of DATS on the expression of inflammatory and antiviral cytokines. A549 cells were treated with DATS and cultured in maintenance medium. At 24 h and 48 h after DATS treatment, total RNA was extracted and analyzed for expression levels of inflammatory and antiviral cytokines by real-time PCR assay. GAPDH was used as an internal control. Fold change in gene expression was calculated using the 2−ΔΔCt method. AMT (500 µM) was used as the positive drug control. Abbreviations are the same as those in Fig. 2. Values are averages of three independent examinations. Significant difference was determined by comparing the data to those of H9N2 AIV-infected group (*p < 0.05, **p < 0.01)

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Virology Journal

ISSN: 1743-422X