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Fig. 2 | Virology Journal

Fig. 2

From: Antiviral activity of diallyl trisulfide against H9N2 avian influenza virus infection in vitro and in vivo

Fig. 2

Effect of DATS on H9N2 AIV replication in A549 cells. A549 cells were treated with three different concentrations (375 µM, 187.5 µM, and 93.75 µM) of DATS 24 h before or 1 h after H9N2 AIV infection and then cultured in maintenance medium for 48 h. AMT (500 µM) was used as the positive drug control. a Viability of A549 cells was measured by MTT assay. b Culture supernatants were collected at 24 h and 48 h after infection and assayed for TCID50 on MDCK cells. c RNA was isolated from cell lysates and assayed for expression levels H9N2 AIV M gene by real-time RT-PCR. Abbreviations: control, uninfected cells; H9N2, H9N2 AIV-infected; AMT + H9N2, H9N2 AIV-infected cells treated with 500 μM AMT; DATS + H9N2, H9N2 AIV-infected cells treated with DATS. Values are averages of three independent examinations. Significant difference was determined by comparing the data to those of H9N2 AIV-infected group (*p < 0.05, **p < 0.01)

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Virology Journal

ISSN: 1743-422X