Fig. 1

CPXV-∆p28 replication in different cell lines. Shown are ∆cT values (48 h p.i.) as a measure of viral DNA replication in cells infected (MoI = 0.1) with the p28 knockout virus CPXV-∆p28, the corresponding revertant virus and wild-type CPXV (n = 2). ∆cT values were obtained via normalization to expression of the cellular MYC gene. All cell lines were obtained from American Type Culture Collection (ATCC) and routinely screened for mycoplasma contamination. THP-1 cells were stimulated with PMA before infection. Statistics: two-way ANOVA and Tukey’s multiple comparison test