Fig. 5

Replication kinetics of PUUV infection in human primary pulmonary cells. A Number of infected pulmonary cells was monitored by quantification of cells expressing N protein on day 2, 4 and 6 after inoculation. B Release of infectious particles was quantified via first round of infection assay by inoculation of Vero E6 cells with 10 µl cell-free supernatant (SN) derived from infected pulmonary cells on day 2, 4 and 6 after infection. Initially infected Vero E6 cells were quantified via immunofluorescence staining for N protein after 48 h post infection and the amount of infectious units (IU) per ml calculated. Experiments were performed in triplicates. Shown is mean ± standard deviation (SD). Differences between donors in mean percentage of infected cells and infectious units were compared using two-tailed Student’s t-test. P values of < 0.05 were considered significant. *P < 0.05; **P < 0.005; ***P < 0.0005; ****P < 0.0001; ns not significant