Fig. 3
Analysis of human pulmonary cells from second donors for permissiveness to PUUV infection. A Detection of marker proteins CD31 and cytokeratin 18 and receptor integrin αVβ3 by immunofluorescence (scale bar: 100 µm). B Pulmonary cells were inoculated with PUUV and analyzed for N protein expression on day 6 post infection. Cells were analyzed by immunofluorescence (scale bar: 100 µm). In lysates and cell-free supernatants (SN), N protein expression was detected by Western blot