Fig. 2

Infection of primary human pulmonary epithelial cells with PUUV. A Detection of marker proteins CD31, cytokeratin 18 and orthohantaviral receptor integrin α_Vβ₃ (scale bar: 100 µm). B HBEpCs, HSAEpCs, and HPAEpiCs were infected with PUUV. Infection and release were monitored by detection of N protein via immunofluorescence and via Western blot analysis of lysates and cell-free supernatants (SN) on day 6 post infection (scale bar: 100 µm). C Vero E6 cells were incubated with cell-free supernatants of uninfected or PUUV-infected pulmonary epithelial cells (6 dpi) and stained for N protein after 48 h post inoculation (scale bar: 100 µm)