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Fig. 1 | Virology Journal

Fig. 1

From: Cells of the human respiratory tract support the replication of pathogenic Old World orthohantavirus Puumala

Fig. 1

Infection of HPMECs with PUUV. A HPMECs were stained for CD31, cytokeratin 18 and integrin αVβ3 (scale bar: 100 µm). B Cells were infected with PUUV and analyzed for expression of N protein by immunofluorescence on day 6 post infection (scale bar: 100 µm). Cellular lysates and cell-free supernatants (SN) were examined for N protein by Western blot analysis on day 6 post infection. C Vero E6 cells were incubated with cell-free supernatants derived from uninfected or infected HPMECs (6 dpi) and analyzed for N protein expression after 48 h post infection (scale bar: 100 µm)

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