First molecular characterization of poxviruses in cattle, sheep, and goats in Botswana

Table 2 Primers used in this study for the HRM assay and sequencing

Method	Primer name	Primer sequence	Amplicon size	Target and references
HRM	CaPV-HRM-For	TCCTGGCATTTTAAGTAATGGT	100	Capripoxviruses [15]
	CaPV-HRM-Rev	GTCAGATATAAACCCGGCAAGTG	100	Capripoxviruses [15]
	PPV-HRM-For	TCGAAGATCTTGTCCAGGAAG	112	Parapoxviruses [15]
	PPV-HRM-Rev	CCGAGAAGATCAACGAGGTC	112	Parapoxviruses [15]
	OPV-HRM-For	AGGACTAGCCGCGGTAACTTT	56	Orthopoxviruses [15]
	OPV-HRM-Rev	ACAAGATAGAAGCGATGGATACTT	56	Orthopoxviruses [15]
Sequencing	CpGPCR-OL1F	TGAAAAATTAATCCATTCTTCTAAACA	617	Capripoxviruses [18]
	CpGPCR-OL1R	TCATGTATTTTATAACGATAATGCAAA	617
	CpGPCR-OL2F	TTAGCGGTATAATCATTCCAAATA	603
	CpGPCR-OL2R	GCGATGATTATGATGATTATGAAGTG	603
	CpGPCR-OL3F	CACAATTATATTTCCAAATAATCCAA	684
	CpGPCR-OL3R	TGTACATGTGTAATTTTAATGTTCGTA	684
	CpRPO30-OL1F	CAGCTGTTTGTTTACATTTGATTTTT	554
	CpRPO30-OL1R	TCGTATAGAAACAAGCCTTTAATAGA	554
	CpRPO30-OL2F	TTTGAACACATTTTATTCCAAAAAG	520
	CpRPO30-OL2R	AACCTACATGCATAAACAGAAGC	520
	ORFV-B2Lf-For	GACCTTCCGCGCTTTAATTT	1210	Parapoxviruses [19]
	ORFV-B2Lf-Rev	CCCGCCTGCTAAAAGACT	1210	Parapoxviruses [19]

ISSN: 1743-422X