Fig. 4

Comparison of ZIKA–EGFP and ZIKA–WT toxic effects cells. a SH-SY5Y cells were incubated with ZIKA–WT and ZIKA–EGFP (MOI = 1.0), and cell viability was determined by CCK-8 assay at 48- and 72-h post-infection. b Cytopathic effect was compared by plaque assays with Vero cells. c Fluorescent cells aggregated due to the cytopathic effects associated with ZIKA–EGFP in Vero cells. CCK-8 tests were repeated three times and each group contained six replicates. **P < 0.01, ***P < 0.001