Molecular detection and identification of Culex flavivirus in mosquito species from Jeju, Republic of Korea

Table 1 Polymerase chain reaction (PCR) conditions for the detection of flavivirus

Flavivirus Target gene	Primer name	Nucleotide sequence (5′-3′)	Product size (bp)	PCR protocol (˚C/s)				Reference
Flavivirus Target gene	Primer name	Nucleotide sequence (5′-3′)	Product size (bp)	Denaturation	Annealing	Extension	Cycles	Reference
NS5	PanF-NS5-1373F PanF-NS5-2481R	AACATGATGGGVAARAGAGAG ATCCACACHCKGTTCCASAC	1108	94/60	52/60	72/60	35	In-house design
NS5	FL-F1 FL-R3 FL-R4	GCCATATGGTACATGTGGCTGGGAGC GTKATTCTTGTGTCCCAWCCGGCTGTGTCATC GTGATGCGRGTGTCCCAGCCRGCKGTGTCATC	212	94/30	58/30	72/30	30	[14]

ISSN: 1743-422X