Table 1 Sequences of primers used in this study
Name | Sequence (5′-3′) | Purpose | References |
|---|---|---|---|
HPV16 E6_F47R.Fw | GGTATATGACTTTGCTCGTCGGGATTTATGC | defective for polyubiquitination | [48] |
HPV16 E6_F47R.Rv | GCATAAATCCCGACGAGCAAAGTCATATACC | defective for polyubiquitination | [48] |
HPV16 E6_C106R.Fw | GGTGTATTAACCGTCAAAAGCCACTG | p53 binding mutant | [47] |
HPV16 E6_C106R.Rv | CAGTGGCTTTTGACGGTTAATACACC | p53 binding mutant | [47] |
HPV16 E7_AGQ.Fw | CCAGAGACAACTGATGCCTACGGTTATCAGCAATTAAATGACAGC | pRb binding mutant | [49] |
HPV16 E7_AGQ.Rv | GCTGTCATTTAATTGCTGATAACCGTAGGCATCAGTTGTCTCTGG | pRb binding mutant | [49] |
HPV16 E7_GSE.Fw | GTAACCTTTGGTAGCGAGTGTGACTCTACG | CxxC mutant | [12] |
HPV16 E7_GSE.Rv | CGTAGAGTCACACTCGCTACCAAAGGTTAC | CxxC mutant | [12] |
sT_R7A.Fw | AGTCCTAAATGCGAAAGAAAGAGAGGC | PP2A binding mutant | [45] |
sT_R7A.Rv | GCCTCTCTTTCTTTCGCATTTAGGACT | PP2A binding mutant | [45] |
sT/LT_D44N.Fw | GCATCACCCTAATAAAGGGGGAAATCC | DnaJ binding mutant | [45] |
sT/LT_D44N.Rv | GGATTTCCCCCTTTATTAGGGTGATGC | DnaJ binding mutant | [45] |
sT_L142A.Fw | GCAAAAAAACTGTGCGACGTGGGGAGAG | PP2A binding mutant | [45] |
sT_L142A.Rv | CTCTCCCCACGTCGCACAGTTTTTTTGC | PP2A binding mutant | [45] |
sT_91AAAAA95.Fw | CCTTGGGAAGAATATGGAACTGCAGCGGCTGCTGCGCAAAGTGGATATAATGCTAG | Fbxw7 binding mutant | [45] |
sT_91AAAAA95.Rv | CTAGCATTATATCCACTTTGCGCAGCAGCCGCTGCAGTTCCATATTCTTCCCAAGG | Fbxw7 binding mutant | [45] |
LT_W209A.Fw | CGTATGGCACCGCGGAGGATCTCTTCTGC | hVam6p mutant | [44] |
LT_W209A.Rv | GCAGAAGAGATCCTCCGCGGTGCCATACG | hVam6p mutant | [44] |
LT_E216K.Fw | GGATCTCTTCTGCGATAAATCACTTTCCTCCCCTGAG | pRb binding mutant | [44] |
LT_E216K.Rv | CTCAGGGGAGGAAAGTGATTTATCGCAGAAGAGATCC | pRb binding mutant | [44] |
HPV16LCR Δ1_Fw | GTGTATATGTTTGTATGAGCTCGTATGTGCTTG | Truncated HPV16 LCR | This study |
HPV16LCRΔ1_Rv | CAAGCACATACGAGCTCATACAAACATATACAC | Truncated HPV16 LCR | This study |
HPV16LCRΔ2_Fw | CACCTACTAATTGAGCTCTGGTTATTCATTG | Truncated HPV16 LCR | This study |
HPV16LCRΔ2_Rv | CAATGAATAACCAGAGCTCAATTAGTAGGTG | Truncated HPV16 LCR | This study |
HPV16LCRΔ3_Fw | CCTGACCTGCAGAGCTCGCCAACCATTCC | Truncated HPV16 LCR | This study |
HPV16LCRΔ3_Rv | GGAATGGTTGGCGAGCTCTGCAGGTCAGG | Truncated HPV16 LCR | This study |
HPV18LCRΔ1_Fw | GTCCTGTGTTTGAGCTCGTTGTATGATTGC | Truncated HPV18 LCR | This study |
HPV18LCRΔ1_Rv | GCAATCATACAACGAGCTCAAACACAGGAC | Truncated HPV18 LCR | This study |
HPV18LCRΔ2_Fw | CCTCCATTTTGAGCTCCAACCGATTTCG | Truncated HPV18 LCR | This study |
HPV18LCRΔ2_Rv | CGAAATCGGTTGGAGCTCAAAATGGAGG | Truncated HPV18 LCR | This study |
HPV18LCRΔ3_Fw | CCTGTCCAGGTGAGCTCCAACAATTGCTTGC | Truncated HPV18 LCR | This study |
HPV18LCRΔ3_Rv | GCAAGCAATTGTTGGAGCTCACCTGGACAGG | Truncated HPV18 LCR | This study |
MCPyV-EΔ1_Fw | GTTTATCAGTCGAGCTCCGCCTCTCC | Truncated early MCPyV promoter | This study |
MCPyV-EΔ1_Rv | GGAGAGGCGGAGCTCGACTGATAAAC | Truncated early MCPyV promoter | This study |
MCPyV-EΔ2_Fw | GGCAGTATCTAAGGGGAGCTCCCAAGGGC | Truncated early MCPyV promoter | This study |
MCPyV-EΔ2_Rv | GCCCTTGGGAGCTCCCCTTAGATACTGCC | Truncated early MCPyV promoter | This study |
MCPyV-LΔ1_Fw | CAGAGGCCTCGGAGCTCAGGAGCCCCAAGC | Truncated late MCPyV promoter | This study |
MCPyV-LΔ1_Rv | GCTTGGGGCTCCTGAGCTCCGAGGCCTCTG | Truncated late MCPyV promoter | This study |
MCPyV-LΔ2_Fw | CCTGGAGAGGCGGAGCTCGACTGATAAACAAAAC | Truncated late MCPyV promoter | This study |
MCPyV-LΔ2_Rv | GTTTTGTTTATCAGTCGAGCTCCGCCTCTCCAGG | Truncated late MCPyV promoter | This study |