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Fig. 7 | Virology Journal

Fig. 7

From: Reciprocal transactivation of Merkel cell polyomavirus and high-risk human papillomavirus promoter activities and increased expression of their oncoproteins

Fig. 7

Effect of E6 and E7 on the transcriptional activity of MCPyV early and late promoter deletion mutants in C33A cells. A Schematic presentation of the full length and truncated MCPyV early and late promoter. The non-coding region (NCCR) consists of 464 bp was cloned in late to early direction upstream of the luciferase gene (= early MCPyV promoter) or in the early to late direction upstream of the luciferase gene (= late MCPyV promoter). The number of nucleotides in the promoter is given in parenthesis. B Cells were co-transfected with 400 ng luciferase reporter plasmid containing the MCPyV early (respectively late) promoter or truncated versions and 400 ng of expression plasmids for E6 or E7. The figure in the left panel shows transactivation by E6 or E7. The transcriptional activity of the early (respectively late) promoter in the presence of empty vector pcDNA3.1 (EV) was arbitrary set as 100%. The figure in the right panel represents the activity of early promoter (respectively late promoter) and its truncated versions. The activity of the full-length MCPyV early promoter (respectively late promoter) was arbitrary set as 100%. Luciferase activity was corrected for the protein concentration in the lysate. Each bar represent the average of three independent parallels ± SD. Similar results were obtained in an independent experiment. *p < 0.05; **p < 0.01; ***p < 0.001

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Virology Journal

ISSN: 1743-422X