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Fig. 6 | Virology Journal

Fig. 6

From: Reciprocal transactivation of Merkel cell polyomavirus and high-risk human papillomavirus promoter activities and increased expression of their oncoproteins

Fig. 6

Effect of MCPyV LT and sT on the transcriptional activity of HPV16 LCR and HPV18 LCR mutants in C33A cells. A Schematic presentation of the truncated HPV16 and HPV18 LCR mutants. An additional SacI site was introduced by site-directed mutagenesis in the luciferase reporter plasmid containing the HPV16 LCR or the HPV18 LCR. The mutated plasmid was then cut with SacI and religated, resulting in truncation of the distal sequences of the LCR. The number of nucleotides in the LCR is given in parenthesis. B Cells were co-transfected with 400 ng luciferase reporter plasmid containing the HPV16 LCR or truncated versions and 400 ng of expression plasmids for LT or sT. The figure in the left panel shows transactivation by LT or sT. The transcriptional activity of the early (respectively late) promoter in the presence of empty vector pcDNA3.1 (EV) was arbitrary set as 100%. The figure in the right panel represents the activity of HPV16 LCR and its truncated versions. The activity of the full-length HPV16 LCR was arbitrary set as 100%. C As in (B) but with the luciferase reporter plasmid with HPV18 LCR or truncated versions. Luciferase activity was corrected for the protein concentration in the lysate. Each bar represent the average of three independent parallels ± SD. Similar results were obtained in an independent experiment. *p < 0.05; **p < 0.01; ***p < 0.001

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