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Fig. 5 | Virology Journal

Fig. 5

From: Reciprocal transactivation of Merkel cell polyomavirus and high-risk human papillomavirus promoter activities and increased expression of their oncoproteins

Fig. 5

Effect of mutations in E6 and E7 on their ability to transactivate the early and late promoter of MCPyV. C33A cells were transfected with 400 ng luciferase reporter plasmid containing either the early or the late promoter of MCPyV. Co-transfection was done with 400 ng of empty expression vector (EV) or expression plasmids for wild-type LT, wild-type sT or their mutants. Luciferase activity was corrected for the protein concentration in the lysate. Each bar represents the average of three independent parallels ± SD. The transcriptional activity of the early (respectively late) promoter in the presence of empty vector pcDNA3.1 (EV) was arbitrary set as 100%. Similar results were obtained in an independent experiment. *p < 0.05; **p < 0.01; ***p < 0.001

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