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Fig. 4 | Virology Journal

Fig. 4

From: Reciprocal transactivation of Merkel cell polyomavirus and high-risk human papillomavirus promoter activities and increased expression of their oncoproteins

Fig. 4

Effect of LT and sT mutants on HPV16 and HPV18 LCR activity. A MCPyV MKL-1 and MKL-2 truncated LT stimulate the transcriptional activity of the HPV16 LCR and HPV18 LCR in C33A cells. B Effect of MCPyV LT and sT mutants on the transcriptional activity of the HPV16 LCR. C Effect of MCPyV LT and sT mutants on the transcriptional activity of the HPV18 LCR. Cells were transfected with 400 ng luciferase reporter plasmid and 400 ng of expression plasmids for MCPyV full-length LT, MKL-1 LT, MKL-2 LT, LT mutants, or sT mutants. Luciferase activity was corrected for the protein concentration in the lysate. Each bar represents the average of three independent parallels ± SD. The transcriptional activity of the early (respectively late) promoter in the presence of empty vector pcDNA3.1 (EV) was arbitrary set as 100%. Similar results were obtained in an independent experiment. *p < 0.05; **p < 0.01; ***p < 0.001

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Virology Journal

ISSN: 1743-422X