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Fig. 6 | Virology Journal

Fig. 6

From: Zoonotic vaccinia virus strains belonging to different genetic clades exhibit immunomodulation abilities that are proportional to their virulence

Fig. 6

Activated phenotype in CD8 + T-cell and B lymphocyte from BALB/c mice uninfected or infected with VACV-BR. Groups of mice (n = 7) were inoculated with PBS or 106 PFU of PSTV, GP1V or VACV-WR by the intranasal route. The mean fluorescence intensity of CD8 T lymphocytes expressing CD28 (A) surface activation marker and percentage of B lymphocytes CD19 expressing CD80 (B) or CD86 (C) was determined 7 days post infection. The cells stained with monoclonal antibodies linked to fluorochromes against cell surface molecules were detected by flow cytometry. The error bars indicate the standard deviations. Significance variations were calculated using the One-way ANOVA post hoc Bonferroni with statistical significance: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. During the experiment some animals were lost (The local Committee of Ethics for Animal Experimentation does not allow the replacement of animals to compensate for this loss)

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