Fig. 4

Proliferation of splenocytes from BALB/c mice uninfected or infected with VACV-BR. Groups of mice (n = 7) were inoculated with PBS or 10⁶ PFU of PSTV, GP1V or VACV-WR by the intranasal route. The spleen cells proliferation was determined 7 days post infection with BrdU Cell Proliferation Assay (Millipore, EUA) by measuring the absorbance at 450 nm. For that, spleen cells were cultured in the presence of 2 µg/mL of Concanavalin A (black dots), 10⁴ PFU of UV-inactivated VACV-WR (gray dots) or mock-treated with medium (light brown dots). The absorbance values were plotted. The error bars indicate the standard deviations. Significance variations were calculated using the One-way ANOVA post hoc Bonferroni with statistical significance: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. During the experiment some animals were lost (The local Committee of Ethics for Animal Experimentation does not allow the replacement of animals to compensate for this loss)