Fig. 3
Pathological analysis of liver, spleen and lung of BALB/c mice uninfected or infected with VACV-BR. Groups of mice (n = 7) were inoculated with PBS or 106 PFU of PSTV, GP1V or VACV-WR by the intranasal route. The liver inflammation (A) and degeneration (B), spleen reactivity (C), lung inflammation (D), edema and hemorrhage (E) were determined 7 days post infection. Scores were assigned to severity and distribution of the assessed characteristic on the tissue and these values were converted to a total score. For severity and distribution, the scale adopted scores from 0 to 5 or 0 to 4, in which the maximum value corresponds to a greater severity and distribution of the pathological alteration in the studied tissue. The pathological scores were plotted. The error bars indicate the standard deviations. Significance variations were calculated using the One-way ANOVA post hoc Bonferroni with statistical significance: *p < 0.05, **p < 0.01, ***p < 0.001