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Table 3 Calculated amplicon Tm values of representative non-Japanese isolates

From: One-step real-time multiplex reverse transcription-polymerase chain reaction assay with melt curve analysis for detection of potato leafroll virus, potato virus S, potato virus X, and potato virus Y

Virus

Isolate

Accessionsa

Strain/lineageb

Calculated Tm (°C)

PLRV

PLRV165

MG356502

87.0

PLRV-IM

KC456052

87.0

14.2

AF453394

87.5

D00530

D00530

87.5

GAF318-4.2

KU586454

87.5

Polish isolate

X74789

87.5

PVS

Leona

AJ863509

PVSO

81.0

Dic2

KR152654

PVSP

81.0

BB-AND

JQ647830

PVSA

81.5

RL5

JX683388

PVSA

81.5

Id4106-US

FJ813513

PVSO

82.0

RVC Andean

JX419379

PVSP

82.0

Yunnan YN

KC430335

PVSO

82.5

PVX

X3

D00344

 

84.5

Taiwan

AF272736

84.5

PVX_Tn148

MF682528

84.5

FX21

EF423572

85.0

Iran

FJ461343

85.0

JAL-2

KR605396

85.5

PVY

NC57

DQ309028

PVYC

77.5

Adgen

AJ890348

PVYC

78.0

P141

MT264735

PVYNA-N

78.0

RRA-1

AY884984

PVYNA-N

78.0

PVY-MON

JF928458

PVYE

78.5

PVY-AGA

JF928459

PVYE

79.0

CO11

KY847937

PVYO5

79.0

MT100010

KY847987

PVYO5

79.0

MSU_45_384a

KY847984

PVYEu-N

79.0

MT100017

KY847988

PVYEu-N

79.0

ME_236_120

KY847962

PVYO

79.0

CA14

KY847936

PVYO

79.5

  1. aGenBank accession
  2. bThe classification of the lineage for PVS was according to Vallejo et al. [14], the strains for PVY according to Green et al. [15]