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Table 3 Calculated amplicon Tm values of representative non-Japanese isolates

From: One-step real-time multiplex reverse transcription-polymerase chain reaction assay with melt curve analysis for detection of potato leafroll virus, potato virus S, potato virus X, and potato virus Y

Virus Isolate Accessionsa Strain/lineageb Calculated Tm (°C)
PLRV PLRV165 MG356502 87.0
PLRV-IM KC456052 87.0
14.2 AF453394 87.5
D00530 D00530 87.5
GAF318-4.2 KU586454 87.5
Polish isolate X74789 87.5
PVS Leona AJ863509 PVSO 81.0
Dic2 KR152654 PVSP 81.0
BB-AND JQ647830 PVSA 81.5
RL5 JX683388 PVSA 81.5
Id4106-US FJ813513 PVSO 82.0
RVC Andean JX419379 PVSP 82.0
Yunnan YN KC430335 PVSO 82.5
PVX X3 D00344   84.5
Taiwan AF272736 84.5
PVX_Tn148 MF682528 84.5
FX21 EF423572 85.0
Iran FJ461343 85.0
JAL-2 KR605396 85.5
PVY NC57 DQ309028 PVYC 77.5
Adgen AJ890348 PVYC 78.0
P141 MT264735 PVYNA-N 78.0
RRA-1 AY884984 PVYNA-N 78.0
PVY-MON JF928458 PVYE 78.5
PVY-AGA JF928459 PVYE 79.0
CO11 KY847937 PVYO5 79.0
MT100010 KY847987 PVYO5 79.0
MSU_45_384a KY847984 PVYEu-N 79.0
MT100017 KY847988 PVYEu-N 79.0
ME_236_120 KY847962 PVYO 79.0
CA14 KY847936 PVYO 79.5
  1. aGenBank accession
  2. bThe classification of the lineage for PVS was according to Vallejo et al. [14], the strains for PVY according to Green et al. [15]