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Fig. 3 | Virology Journal

Fig. 3

From: One-step real-time multiplex reverse transcription-polymerase chain reaction assay with melt curve analysis for detection of potato leafroll virus, potato virus S, potato virus X, and potato virus Y

Fig. 3

Confirmation of detection sensitivity for each potato virus. Ten-fold dilution of leaf homogenates was prepared by mixing virus-free and virus-infected potato leaf homogenates with a PLRV (isolate: ChLR_2), b PVSO (M), c PVX (O-IC249), or d PVYNTN (Eu-12Jp). The peaks (melting temperature) for plant EF1α and each virus are shown. Melt curve colors: black, virus-free potato leaf; red, a tenfold dilution of each virus; green, 100-fold of each; blue, 1000-fold

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Virology Journal

ISSN: 1743-422X