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Fig. 2 | Virology Journal

Fig. 2

From: One-step real-time multiplex reverse transcription-polymerase chain reaction assay with melt curve analysis for detection of potato leafroll virus, potato virus S, potato virus X, and potato virus Y

Fig. 2

Melt curve analysis for multiple detections of four potato viruses without the PVS genome or PVS-specific primers. Using singly-virus-infected potato (cv. IC) leaf homogenates, the templates were prepared by mixing a the three RNA solutions of PLRV (isolate: ChLR_2), PVX (O-IC249), and PVYNTN (Eu-12Jp) or from b the mixture of four virus-infected homogenates, PLRV, PVSO (M), PVX, and PVYNTN. The peaks (melting temperature) for plant EF1α and each virus are shown

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