Fig. 2From: One-step real-time multiplex reverse transcription-polymerase chain reaction assay with melt curve analysis for detection of potato leafroll virus, potato virus S, potato virus X, and potato virus YMelt curve analysis for multiple detections of four potato viruses without the PVS genome or PVS-specific primers. Using singly-virus-infected potato (cv. IC) leaf homogenates, the templates were prepared by mixing a the three RNA solutions of PLRV (isolate: ChLR_2), PVX (O-IC249), and PVYNTN (Eu-12Jp) or from b the mixture of four virus-infected homogenates, PLRV, PVSO (M), PVX, and PVYNTN. The peaks (melting temperature) for plant EF1α and each virus are shownBack to article page