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Fig. 2 | Virology Journal

Fig. 2

From: Simple rapid in vitro screening method for SARS-CoV-2 anti-virals that identifies potential cytomorbidity-associated false positives

Fig. 2

Crystal violet staining for remdesivir and hydroxychloroquine. Cytotoxicity assay (Vero E6 seeded at 104 cells/well with no virus). Cytomorbidity assay (Vero E6 seeded at 400 cells/well with no virus). Viral CPE (Vero E6 seeded at 104 cells/well with virus MOI ≈ 0.01). After 4 days in culture 96 well plates were fixed and stained with paraformaldehyde and crystal violet, respectively. Plates were washed in water, dried and scanned, and for the data in Fig. 1, the dye was dissolved in methanol and read at OD595 nm. For the Cytotoxicity assay wells encircled in red show overt cytotoxicity. For the Cytomorbidity assay wells encircled in red show overt cell growth reduction. For viral CPE assay, wells encircled in red show inhibition of CPE indicating potential antiviral activity

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