Fig. 4

Effect of MBZM-N-IBT in HSV-1 protein levels: Vero cells were infected with HSV-1 at MOI 1 and subsequently treated with DMSO (negative control), Acyclovir (positive control) and different concentration of MBZM-N-IBT (100 µM, 150 µM and 200 μM) and finally harvested at 24 hpi for ICP8 and 15 hpi for gC. The cells were lysed and subjected to Western blot analysis. Western blots were probed with ICP8 (a) and gC (b) antibodies. GAPDH was used as a loading control and the band intensities were normalized based on GAPDH by the ImageJ software. c, d Bar diagrams showing the relative band intensities of ICP8 and gC. p ≤ 0.05 was considered statistically significant. e Flowcytometric dot plot analysis showing percent positive cells for ICP8 at 15 hpi against SSC-H off different samples. f Graphical representation showing percent positive cells for ICP8 with varying concentrations of MBZM-N-IBT. g Mean fluorescence intensity (MFI) analysis also suggests the ICP8 signal intensity in the presence of MBZM-N-IBT in a dose dependent manner as compared to DMSO control. The comparison between the groups with only one parameter was performed by one way ANOVA (nonparametric) with the Bonferroni post-hoc test. p < 0.05 was considered as the statistically significant difference between the groups. (ns, non-significant; ** p ≤ 0.01; *** p ≤ 0.001)