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Fig. 3 | Virology Journal

Fig. 3

From: Inhibition of herpes simplex virus-1 infection by MBZM-N-IBT: in silico and in vitro studies

Fig. 3

Effect of MBZM-N-IBT in HSV-1 mRNA and DNA levels: a Vero cells were infected by HSV-1 with MOI 1 and treated with DMSO (negative control), 44.4 µM Acyclovir (positive control) and MBZM-N-IBT (100 µM, 150 µM, 200 µM,). Total RNA was extracted from the HSV-1 infected cells at 24 hpi, cDNA was synthsized and HSV-1 UL9, ICP8, gC, gD genes were amplified using respective primers by RT-PCR (a) Bar diagrams (be) depicts the relative band intensities of viral mRNA expression pattern in infected and drug treated samples of UL9, ICP8, gC and gD genes respectively. Data represent the mean ± SEM from three independent experiments using the one way Anova, Dunnett’s multiple comparison tests.. p ≤ 0.05 was considered statistically significant. GAPDH was used as a loading control and the band intensities were normalized based on GAPDH by the ImageJ software. f Total RNA was isolated from the HSV-1 infected cells at 24 hpi, cDNA was synthesized and the expression levels of HSV-1 UL9 and gC mRNA, were detected using respective primers by qRT-PCR. Bar diagrams showing the relative fold change reduction in viral mRNA expression pattern in infected and drug treated samples. Data represent the mean ± SEM from three independent experiments using the one way Anova, Dunnett’s multiple comparisons test. p ≤ 0.05 was considered statistically significant. GAPDH was used as endogenous control. g Vero cells were infected by HSV-1 with MOI 1 and treated with DMSO (negative control) and MBZM-N-IBT (50, 100, 200 µM). Cell supernatants were collected at 24hpi and HSV-1 DNA was isolated by the Qiagen All prep DNA/RNA Mini kit according to the manufacturer’s protocol followed by qPCR of HSV-1 gC gene. Bar diagram represents the viral copy number in percentage which was calculated based on standard curve. Data represent the mean ± SEM from three independent experiments using the one way Anova, Dunnett’s multiple comparisons test

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