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Fig. 7 | Virology Journal

Fig. 7

From: Development of a triple antibody sandwich enzyme-linked immunosorbent assay for cassava mosaic disease detection using a monoclonal antibody to Sri Lankan cassava mosaic virus

Fig. 7

SLCMV genome copy number quantification by qPCR. A serial tenfold dilution of the plasmid standard (pGEM-T Easy vector containing a 2.5-kb fragment of SLCMV DNA-A) ranging from 3.0 × 108 to 30 copies per 5 µl was prepared and used for quantitative analyses by qPCR. A linear regression line with a PCR amplification efficiency of 92.4% and a correlation coefficient of 0.999 was obtained and used to determine the copy number of SLCMV-DNA-A in the test samples. All samples were assayed in triplicate. Data and statistical analyses were done using the CFX Manager Software version 3.1 (BIO-RAD, USA). Circle “ο” indicates Cq from each replicates of standard plasmid, cross mark “×” indicates Cq from each replicates of three test samples

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