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Fig. 4 | Virology Journal

Fig. 4

From: Development of a triple antibody sandwich enzyme-linked immunosorbent assay for cassava mosaic disease detection using a monoclonal antibody to Sri Lankan cassava mosaic virus

Fig. 4

Specificity analysis of MAb 29B3 against SLCMV and select begomoviruses by western blot analysis. Protein lysates prepared from sap extracts of healthy cassava or sap extracts of plants infected with SLCMV and different types of begomoviruses, partially purified SLCMV, recombinant SLCMV-CP and ICMV-CP protein were subjected to western blotting and probing with MAb 29B3. Lane 1: healthy cassava, Lane 2: SLCMV-infected cassava, Lane 3: AYVV-infected Ageratum conyzoides, Lane 4: PepYLCIV-infected pepper, Lane 5: TYLCKaV-infected eggplant, Lane 6: SLCCNV-infected pumpkin, Lane 7: ToLCNDV-infected cucurbits, Lane 8: PepYLCTHV-infected pepper, Lane 9: TYLCTHV-infected tomato, Lane 10: partially purified SLCMV, Lane 11: recombinant SLCMV-CP, Lane 12: recombinant ICMV-CP, Lane M: PageRuler™ Prestained Protein Ladder (Thermo Scientific, USA). Each lane was loaded with 5 μl of protein extracts from plant sap, 10 μg of a partially purified virus or 0.1 μg of recombinant coat protein. The size of the coat protein in plant extract and partially purified virus (31 kDa) and the recombinant coat protein (34 kDa) are indicated

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