Fig. 1
From: Molecular-genetic characterization of human parvovirus B19 prevalent in Kerala State, India
Analysis of DNA fragments obtained by nested PCR on the unique conserved regions of VP1/2 gene of human B19V. Parvovirus B19V PCR and amplified products were electrophoresed in a 1% agarose-TAE gel and stained with ethidium bromide solution for visualizing using the E-gel imager (Life technologies, USA) gel documentation system. Lane 2 and 3: 284 and 103 bp amplified products of NS1 gene; Lane 4–13: 227, 138, 445, 322, 671, 111, 563, 476, 698 and 610 bp amplified products of VP1/2 gene; Lane 1 and 14 100 bp DNA ladder; lane 15 negative patient sample and 16 negative control. Hepatitis B virus positive clinical samples were used as a negative control for testing primer specificity