Fig. 3From: Development of an indirect ELISA to specifically detect antibodies against African swine fever virus: bioinformatics approachesPreparation of recombinant protein. SDS-PAGE analysis of m35 protein a (M, marker; Lane 1, uninduced cells; Lane 2, IPTG-induced cells for 4 h) b (M, marker; Lane 3, the supernatant of IPTG-induced cells; Lane 4, deposition of IPTG-induced cells) c (M, marker; Lane 5, purified recombinant protein) Western blotting analysis of the purified recombinant protein using the mouse anti‐His monoclonal antibody (mAb) (d) and the ASFV-positive serum (e)Back to article page