Table 2 Results of WHO EQA specimens obtained by direct RT-qPCR and classic RT-qPCR approaches during WHO (2020) EQA Testing Program for the Detection of SARS-CoV-2 by PCR in February 2021
WHO EQA specimens ID | Direct-RT-qPCR result | Two-step RT-qPCR result | ||||
|---|---|---|---|---|---|---|
SARS-CoV-2 result | N1/N2 (Ct value) | SARS-CoV-2 result | N gene (Ct value) | E gene (Ct value) | ORF1ab (Ct value) | |
WHO-SC-20-01 | Positive | 27.7 | Positive | 30.5 | 30.1 | 29.2 |
WHO-SC-20-02 | Positive | 23.1 | Positive | 25.8 | 25.3 | 24.1 |
WHO-SC-20-03 | Negative | ND | Negative | ND | ND | ND |
WHO-SC-20-04 | Negative | ND | Negative | ND | ND | ND |
WHO-SC-20-05 | Positive | 32.3 | Positive | 34.0 | 34.6 | 32.5 |