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Table 2 Results of WHO EQA specimens obtained by direct RT-qPCR and classic RT-qPCR approaches during WHO (2020) EQA Testing Program for the Detection of SARS-CoV-2 by PCR in February 2021

From: Excellent option for mass testing during the SARS-CoV-2 pandemic: painless self-collection and direct RT-qPCR

WHO EQA specimens ID Direct-RT-qPCR result Two-step RT-qPCR result
SARS-CoV-2 result N1/N2 (Ct value) SARS-CoV-2 result N gene (Ct value) E gene (Ct value) ORF1ab (Ct value)
WHO-SC-20-01 Positive 27.7 Positive 30.5 30.1 29.2
WHO-SC-20-02 Positive 23.1 Positive 25.8 25.3 24.1
WHO-SC-20-03 Negative ND Negative ND ND ND
WHO-SC-20-04 Negative ND Negative ND ND ND
WHO-SC-20-05 Positive 32.3 Positive 34.0 34.6 32.5
  1. Direct RT-qPCR was performed by heat lysed swab specimen using DIOS-RT-qPCR Kit (IABio, Czechia) and two-step RT-qPCR using Novel Coronavirus (2019-nCoV) Real-Time Multiplex RT-PCR Kit (LifeRiver, Shanghai, China) using extracted RNA from swab specimen
  2. ND not detected