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Fig. 3 | Virology Journal

Fig. 3

From: Excellent option for mass testing during the SARS-CoV-2 pandemic: painless self-collection and direct RT-qPCR

Fig. 3

Inhibition of SARS-CoV-2 virus detection by direct RT-qPCR tested with titrated copies of viable virus culture either spiked immediately into COVID media (orange lines) or spiked into COVID media with nasal swabs from COVID-19-negative patients (blue lines). The live SARS-CoV-2 virus culture was spiked at the following concentrations: 32, 16, 8, 4, 2 and 0 copies/reaction (which translates into 1143, 571, 286, 143 and 0 copies/ml). The lowest concentrations were analysed in triplicate. The data are presented as a amplification curves for particular SARS-CoV-2 concentrations and b the corresponding Ct values. Ct: cycle threshold; SD: standard deviation; Delta Ct: the difference between the COVID medium alone and the COVID medium with negative nasal swabs for the same viral load. *A Ct difference of around 3.3 cycles corresponds to every 1log10 copies/ml change in viral load detection (2^3.3 = 9.48)

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Virology Journal

ISSN: 1743-422X