Table 4 Master mix PCR reaction 2 orf1ab/c-myc
From: Resource-efficient internally controlled in-house real-time PCR detection of SARS-CoV-2
Reagent | Vol. (µL) |
|---|---|
Water | 3.9 |
2× RT-PCR buffer | 12.50 |
orf1ab S (10 µM) | 0.75 |
orf1ab A (10 µM) | 0.75 |
orf1ab TMGB (10 µM) | 0.25 |
c-myc F (10 µM) | 0.3 |
c-myc R (10 µM) | 0.3 |
c-myc TM Cy5 (10 µM) | 0.25 |
25× RT-PCR Enzyme mix | 1.00 |
Mix vol | 20.00 |
Sample vol | 5.00 |
Total vol | 25.00 |