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Table 4 Master mix PCR reaction 2 orf1ab/c-myc

From: Resource-efficient internally controlled in-house real-time PCR detection of SARS-CoV-2

Reagent Vol. (µL)
Water 3.9
2× RT-PCR buffer 12.50
orf1ab S (10 µM) 0.75
orf1ab A (10 µM) 0.75
orf1ab TMGB (10 µM) 0.25
c-myc F (10 µM) 0.3
c-myc R (10 µM) 0.3
c-myc TM Cy5 (10 µM) 0.25
25× RT-PCR Enzyme mix 1.00
Mix vol 20.00
Sample vol 5.00
Total vol 25.00
  1. Volumes used for reaction set up, concentrations of stock solutions are given in brackets