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Table 2 Primers and probes for PCR 2: orf1ab/c-myc

From: Resource-efficient internally controlled in-house real-time PCR detection of SARS-CoV-2

Name

Sequence

Oa

Positionb

Tmc

orf1ab S

CTCTggAACACTTTTACAAgACTTC

S

19,608

54.5

orf1ab A

ACCATCAACTTTTgTgTAAACAgTg

A

19,754

56.3

orf1ab TMGB

FAM-ACAgggTgAAgTACCA-MGB

S

19,688

66.0

c-myc F

TAgTggAAAACCAgCAgCCT

S

380

57.0

c-myc R

TCgTCgCAgTAgAAATACgg

A

488

56.0

c-myc TM

Cy5-TATgACCTCgACTACgACTCggTgC-BBQ

S

442

63.5

  1. aOrientation
  2. bPosition in GenBank entry (orf 1ab: Acc# MN997409.1; c-myc NM_002467.6, GeneID: 4609)
  3. cThermodynamic melting temperature
  4. *Corman et al. [6]
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Virology Journal

ISSN: 1743-422X