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Table 2 Primers and probes for PCR 2: orf1ab/c-myc

From: Resource-efficient internally controlled in-house real-time PCR detection of SARS-CoV-2

Name Sequence Oa Positionb Tmc
orf1ab S CTCTggAACACTTTTACAAgACTTC S 19,608 54.5
orf1ab A ACCATCAACTTTTgTgTAAACAgTg A 19,754 56.3
orf1ab TMGB FAM-ACAgggTgAAgTACCA-MGB S 19,688 66.0
c-myc F TAgTggAAAACCAgCAgCCT S 380 57.0
c-myc R TCgTCgCAgTAgAAATACgg A 488 56.0
c-myc TM Cy5-TATgACCTCgACTACgACTCggTgC-BBQ S 442 63.5
  1. aOrientation
  2. bPosition in GenBank entry (orf 1ab: Acc# MN997409.1; c-myc NM_002467.6, GeneID: 4609)
  3. cThermodynamic melting temperature
  4. *Corman et al. [6]