Table 1 Primers and probes for PCR 1: E-Gene/KoMa
From: Resource-efficient internally controlled in-house real-time PCR detection of SARS-CoV-2
Name | Sequence | Oa | Positionb | Tmc |
|---|---|---|---|---|
E_Sarbeco_F1* | ACAggTACgTTAATAgTTAATAgCgT | S | 26,141 | 53.9 |
E_Sarbeco_R2* | ATATTgCAgCAgTACgCACACA | A | 26,232 | 57.9 |
E_Sarbeco_P1* | FAM-ACACTAgCCATCCTTACTgCgCTTCg-BHQ1 | S | 26,204 | 65.0 |
KoMa F | ggTgATgCCgCATTATTACTAgg | S | n/ad | 57.8 |
KoMa R | ggTATTAgCAgTCgCAggCTT | A | n/ad | 57.8 |
KoMa TM | TexRed-TTCTTgCTTgAggATCTgTCgTggATCg-BBQ | S | n/ad | 67.7 |