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Table 1 Primers and probes for PCR 1: E-Gene/KoMa

From: Resource-efficient internally controlled in-house real-time PCR detection of SARS-CoV-2

Name Sequence Oa Positionb Tmc
E_Sarbeco_F1* ACAggTACgTTAATAgTTAATAgCgT S 26,141 53.9
E_Sarbeco_R2* ATATTgCAgCAgTACgCACACA A 26,232 57.9
E_Sarbeco_P1* FAM-ACACTAgCCATCCTTACTgCgCTTCg-BHQ1 S 26,204 65.0
KoMa F ggTgATgCCgCATTATTACTAgg S n/ad 57.8
KoMa R ggTATTAgCAgTCgCAggCTT A n/ad 57.8
KoMa TM TexRed-TTCTTgCTTgAggATCTgTCgTggATCg-BBQ S n/ad 67.7
  1. aOrientation
  2. bPosition in GenBank entry (E_Sarbeco: Acc# NC_004718)
  3. cThermodynamic melting temperature
  4. dArtificial sequence
  5. *Corman et al.