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Fig. 3 | Virology Journal

Fig. 3

From: SARS-CoV-2 in hospital indoor environments is predominantly non-infectious

Fig. 3

The effects of RNase treatment on the collected environmental samples in the Infectious Disease ward at the Uppsala University Hospital. a The difference in Ct values before and after RNase treatment as well as b the change in detected SARS-CoV-2 copy numbers is displayed. Individual values for N and E gene Ct values and copy numbers are given on top of the columns. Three floor swabs samples and two shoe samples were selected (based on low Ct values) for the analysis. The positive control was an infectious patient isolate of SARS-CoV-2, isolated in our laboratory, and the negative control the extracted isolate RNA. No significant change in Ct value or copy numbers was observed in the infectious virus control, corresponding to the presence of intact nucleocapsids or whole virus particles inhibiting the RNA decay. All analyzed hospital collected samples did likewise not show any significant change in Ct value or copy numbers, indicating protection of the RNA against degradation. The RNA control however was completely degraded after RNase A treatment

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