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Fig. 1 | Virology Journal

Fig. 1

From: Optimization of AAV vectors to target persistent viral reservoirs

Fig. 1

AAV vector biology. a genome organization of wild type AAV2 depicting its ssDNA genome and the 7 viral RNAs expressed from 2 genes Rep (Black boxes) and Cap (Grey boxes) and via the p5, p19 or p40 promoters. b crystal structure of the AAV2 virion (pdb: 1lp3) depicting the fivefold axis of symmetry (left, arrow) and threefold axis of symmetry (right, arrow). The AAV virion contains 60 VP proteins in a 1:1:10 ratio (VP1:VP2:VP3). c production of replication incompetent AAV vectors via transient plasmid transfection into 293 producer cells that express adenovirus type 5 E1 genes. Vectors containing standard or scAAV genomes can be generated following co-transfection of AAV packaging (AAV Rep and Cap containing), adenovirus type 5 helper (E2A, E4 and VA RNA expressing) and AAV vector plasmids without (standard) or with (scAAV) D region deletion in the left ITR

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