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Fig. 1 | Virology Journal

Fig. 1

From: Self-assembled raccoon dog parvovirus VP2 protein confers immunity against RDPV disease in raccoon dogs: in vitro and in vivo studies

Fig. 1

Expression and co-expression of RDPV VP2 protein with Tf16 in E. coli ER2566. a SDS-PAGE analysis of the expression of VP2 protein in E. coli ER2566. Lane M, protein marker; Lane 1, pET30a cells before induced; Lane 2, induced pET30a cells; Lane 3, induced pET30a cells lysate; Lane 4, inclusions of induced pET30a cells; Lane 5, pET30a-VP2 cells before induced; Lane 6, induced pET30a-VP2 cells; Lane 7, induced pET30a-VP2 cells lysate; Lane 8, inclusions of induced pET30a-VP2 cells. b SDS-PAGE analysis of the expression of RDPV VP2 protein co-expressed with Tf16. Lane M, protein marker; Lane 1, pET30a-VP2-Tf16 cells before induced of IPTG; Lane 2, induced pET30a-VP2-Tf16 cells; Lane 3, induced pET30a-VP2-Tf16 cells lysate; Lane 4, inclusions of induced pET30a-VP2-Tf16 cells. c Western blot analysis of the expression of RDPV VP2 protein co-expressed with chaperone Tf16. Lane M, protein marker; Lane 1, pET30a-VP2-Tf16 cells before induced of IPTG; Lane 2, induced pET30a-VP2-Tf16 cells; Lane 3, induced pET30a-VP2-Tf16 cells lysate; Lane 4, inclusions of induced pET30a-VP2-Tf16 cells

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Virology Journal

ISSN: 1743-422X