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Fig. 4 | Virology Journal

Fig. 4

From: Polymerase acidic subunit of H9N2 polymerase complex induces cell apoptosis by binding to PDCD 7 in A549 cells

Fig. 4

PDCD7 mediates the PA-overexpression-induced cell apoptosis in Lung A549 cells. a Western blot analysis of PDCD7 knockout A549 cells, the b-actin was used as internal control. b Flow cytometry analysis of apoptosis in wide type A549 cells or PDCD7 knockout cells upon overexpression with Flag-vector of Flag-PA. c Quantitative results of cells at different stages. Early apoptosis: E-apoptosis; Late apoptosis: L-apoptosis. d Western blot analysis of apoptosis related protein expression in A549 cells or PDCD7 knockout cells upon overexpression with Flag-vector of Flag-PA. e quantitative results of apoptotic related proteins by Image J. “**” means P < 0.01. f Titers of the infected H9N2 virus in wide type A549 cells or PDCD7 knockout A549 cells, “*” means P < 0.05

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