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Fig. 1 | Virology Journal

Fig. 1

From: Polymerase acidic subunit of H9N2 polymerase complex induces cell apoptosis by binding to PDCD 7 in A549 cells

Fig. 1

PDCD7 is a binding target of PA subunit of H9N2 virus. a Immunoprecipitation fractions were separated by SDS-PAGE and stained with Coomassie blue; the exogenous expressed Flag-PA was indicated by arrow. b Western blot detection of PDCD7 in the immunoprecipitation fraction of exogenous expressed Flag-PA; Flag-M2 beads were used to isolate Flag-tagged PA subunit and the Flag-vector was used as negative control. c Western blot detection of His-tagged PDCD7 in the fraction of GST-PA pull-down. The GST protein was used as negative control. d Western blot result showed the endogenous interaction between PDCD7 and PA subunit of the H9N2 virus in A549 cells. The polyclonal antibody for PA was purified from mouse. E. Western blot result shown interaction between PDCD7 and the PA subunit from H5N1. The Flag-vector was used as a negative control

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