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Fig. 1 | Virology Journal

Fig. 1

From: An in vitro model for assessment of SARS-CoV-2 infectivity by defining the correlation between virus isolation and quantitative PCR value: isolation success of SARS-CoV-2 from oropharyngeal swabs correlates negatively with Cq value

Fig. 1

Success of isolation of SARS-CoV-2 in VeroB4 cells by Cq-values (%). Isolation success correlated negatively with the initial Cq value in the sample (r = − 0.76; p < 0.05). All oropharyngeal samples with an initial viral load lower than Cq 20 in E-gene screening PCR (n = 8), could be grown in vitro (100%). An initial Cq value between 20 and 24.9 led to a 72.7% chance of infection, which decreased to 25% at an initial viral load between 25 and 29.9 and to 7.1% at an initial load between 30 and 34.9. Samples with initial viral loads of Cq 35 or higher could not be isolated in our experiment

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