Skip to main content

Table 3 Composition and specificity of the primer–probe (PP) mixes

From: Discriminating the eight genotypes of the porcine circovirus type 2 with TaqMan-based real-time PCR

PP Components (concentration in PCR mix) Specificity
A PCV2a_V1-F (400 nM) + PCV2a_V2-F (400 nM) + PCV2a-R (800 nM) + probe PCV2a (200 nM) PCV2a
B PCV2b-F (800 nM) + PCV2b-R (800 nM) + probe PCV2b (200 nM) PCV2b
C PCV2a_V2-F (800 nM) + PCV2c-R (800 nM) + probe PCV2cgh (200 nM) PCV2c
D PCV2d-F (800 nM) + PCV2d-R (800 nM) + probe PCV2d (200 nM) PCV2d
E PCV2e-F (800 nM) + PCV2e-R (800 nM) + probe PCV2e (200 nM) PCV2e
F PCV2f-F (800 nM) + PCV2f-R (800 nM) + probe PCV2f (200 nM) PCV2f
G PCV2g_V1-F (400 nM) + PCV2g_V2-F (400 nM) + PCV2a-R (800 nM) + probe PCV2cgh (200 nM) PCV2g/2d
H PCV2h_V1-F (400 nM) + PCV2h_V2-F (400 nM) + PCV2a-R (800 nM) + probe PCV2cgh (200 nM) PCV2h/2c