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Table 1 Reactivity of obtained monoclonal antibodies (MAbs)

From: Monoclonal antibodies specific for the hemagglutinin-neuraminidase protein define neutralizing epitopes specific for Newcastle disease virus genotype 2.VII from Egypt

MAb HI (log2) IF ConA ELISA [OD 492] WB SNT (log2) Specificity
NR730 clone 30 NR730 clone 30 NR730 clone 30 NR730 clone 30 NR730
4E11 6 5 pos pos 1.79 1.19 neg n.d 8 HN
5C2 4 neg pos neg 1.69 0.17 neg n.d neg HN
5C9 4 neg pos neg 1.86 0.08 neg n.d 7 HN
5D1 3 neg pos neg 1.74 0.09 neg n.d 8 HN
7A8 5 neg pos neg 1.55 0.07 neg n.d neg HN
7C12 5 neg pos neg 2.04 0.08 neg n.d 8 HN
1B6 neg neg pos neg 0.18 0.18# 55 kD 55 kD neg NP/P*
5B4 neg neg pos neg 0.16 0.06# 55 kD neg neg NP/P*
6F2 neg neg pos neg 0.16 0.05# 55 kD neg neg NP/P*
  1. NR730: virulent Egyptian NDV isolate of genotype 2.VII, directly; clone 30: avirulent NDV vaccine strain of genotype 2.II
  2. (#): directly coated ELISA plates; pos: positive reaction; neg: negative reaction
  3. (*): deduced from western blot analysis due to co-migration of NP and P-protein