Monoclonal antibodies specific for the hemagglutinin-neuraminidase protein define neutralizing epitopes specific for Newcastle disease virus genotype 2.VII from Egypt

Table 1 Reactivity of obtained monoclonal antibodies (MAbs)

MAb	HI (log2)		IF		ConA ELISA [OD 492]		WB		SNT (log2)	Specificity
MAb	NR730	clone 30	NR730	clone 30	NR730	clone 30	NR730	clone 30	NR730	Specificity
4E11	6	5	pos	pos	1.79	1.19	neg	n.d	8	HN
5C2	4	neg	pos	neg	1.69	0.17	neg	n.d	neg	HN
5C9	4	neg	pos	neg	1.86	0.08	neg	n.d	7	HN
5D1	3	neg	pos	neg	1.74	0.09	neg	n.d	8	HN
7A8	5	neg	pos	neg	1.55	0.07	neg	n.d	neg	HN
7C12	5	neg	pos	neg	2.04	0.08	neg	n.d	8	HN
1B6	neg	neg	pos	neg	0.18	0.18^#	55 kD	55 kD	neg	NP/P*
5B4	neg	neg	pos	neg	0.16	0.06^#	55 kD	neg	neg	NP/P*
6F2	neg	neg	pos	neg	0.16	0.05^#	55 kD	neg	neg	NP/P*

NR730: virulent Egyptian NDV isolate of genotype 2.VII, directly; clone 30: avirulent NDV vaccine strain of genotype 2.II
(#): directly coated ELISA plates; pos: positive reaction; neg: negative reaction
(*): deduced from western blot analysis due to co-migration of NP and P-protein

ISSN: 1743-422X