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Fig. 1 | Virology Journal

Fig. 1

From: Monoclonal antibodies specific for the hemagglutinin-neuraminidase protein define neutralizing epitopes specific for Newcastle disease virus genotype 2.VII from Egypt

Fig. 1

Preparation of HN-enriched fraction from purified NDV/NR730/2016. Purified virus and fractions of the preparation were subjected to SDS-PAGE and subsequent Coomassie staining (a) and Western blot analysis using egg yolk preparation of a vaccinated chicken (b), HN specific rabbit hyperimmune serum (c) or F-specific rabbit hyperimmune serum (d). Beside the original gradient purified virus from AAF (V), the discarded pellets after Triton-100 treatment and two successive centrifugation steps (p1 and p2) are shown, together with the insoluble fraction after dialysis (p3) and the final supernatant used for immunisation (s3)

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Virology Journal

ISSN: 1743-422X