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Table 1 sgRNAs used, and their corresponding position within the target open reading frame. Column 1: sgRNA name. Column 2: sgRNA nucleotide position in the orf. Column 3: sgRNA sequence

From: Targeting the Kaposi’s sarcoma-associated herpesvirus genome with the CRISPR-Cas9 platform in latently infected cells

Designation

Position within open reading frame

Sequence

gfp sgRNA 1

329–348

GCGCCGAGGTGAAGTTCGAG

gfp sgRNA 2

563–582

CCATCGGCGACGGCCCCGTG

orf45 sgRNA 1

184–202

CCCAGACGACGTCTTCGCCG

orf45 sgRNA 2

253–272

GATCAGTCGAGCGGCGAATC

orf73 sgRNA 1

113–132

ACCTACATCTACAACCGCGA

orf73 sgRNA 2

143–162

TCGCCGACTCCGTCGACGGC

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Virology Journal

ISSN: 1743-422X