Fig. 3

Viral DNA quantification following CRISPR-Cas9 targeting of gfp, orf45 or orf73. BAC16-mCherry-ORF45-infected SLK cells expressing Cas9 were treated as described in Fig. 1. DNA was extracted and subjected to TaqMan real-time PCR for viral DNA using primers that target the viral orfK6 gene along with the cellular erv-1 gene, which was used to normalize loading. Statistical test was performed between groups including all time points together for each gRNA treatment. *p < 0.05, ***p < 0.001