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Table 2 Ability of VTM to support qPCR

From: Optimal preparation of SARS-CoV-2 viral transport medium for culture

   Swab held at 4 °C Swab held at 18–20 °C
Media Period 0 days 5 days 0 days 5 days
Kang-jian Ct Value ND 28.0 27.1 28.0
MPU VTM Ct Value ND 27.8 28.2 28.0
  1. Quantitative PCR (qPCR) was used to analyse inactivated SARS-CoV-2 was ‘spiked’ into commercially source viral transport media (Kang-Jian) or VTM produced by the Media Production Unit. The transport media were held at 4 °C or RT (18–20 °C) for 5 days, because nucleic acid was extracted and the numbers of genomes determined by cycle threshold (Ct) number in the PCR. The values were within normal variation (i.e. one cycle, 27.5–28.5 cycles) for the amount of nucleic acid inoculated into the transport media. ND: not done