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Fig. 1 | Virology Journal

Fig. 1

From: Construction of a recombinant avipoxvirus expressing the env gene of Zika virus as a novel putative preventive vaccine

Fig. 1

Plasmids construction and in-vivo recombination. Two plasmids were constructed: pFPzenv as the recombination plasmid for in-vivo recombination and pVAXzenv as the expression plasmid to prime the experimental mice. pFPzenv contains the cPrME gene sequence of ZIKV (zenv) obtained by retro-transcription of a 2015 Brazilian isolate. This sequence includes the genes that encode for the tail portion of the capsid protein (c), the membrane precursor (Pr), the membrane protein (M) and the whole envelope protein (E). Homologous in-vivo recombination occurred in specific pathogen-free primary chick embryo cells after infection of FPwt and transfection of the pFPzenv recombination plasmid. Recombinant plaques were identified by autoradiography after hybridization with the [32P]-labelled zenv probe and different positive clones were subjected to multiple cycles of plaque purification until one clone (FPzenv) was selected for correct expression

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